Date of Award
Biology, Animal Physiology.
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In murine muscular dystrophy (strain 129 ReJ), hindlimb muscle contains a functionally defective thiol protease inhibitor (TPI) which has been implicated in the onset and progression of the disease in this strain of mice. More recently, and through the work of several researchers, this protease inhibitor has been identified as parvalbumin, a calcium binding protein. In this study, a polyclonal antibody raised in rabbits against normal mouse muscle parvalbumin (TPI) was used to study the concentration and tissue distribution of this protein in both normal and dystrophic male mice at various ages. Western blotting assays were used to screen extracts of hindlimb, forelimb, brain, heart, lung, liver and kidney in 60 day old normal and dystrophic male mice for parvalbumin content. Parvalbumin was detected in relatively high amounts in both hindlimb and forelimb muscle extracts while much lower concentrations were detected in brain of both normal and dystrophic animals. No parvalbumin was detected in lung, liver, heart or kidney preparations using the above antibody. With aging, the parvalbumin concentration in hindlimb muscle extracts of normal mice was found to remain fairly constant (on a percent protein basis) for 90 days whereupon the levels increased. In contrast, in dystrophic mice the parvalbumin concentration decreased steadily with age to about 22% of control animals at 120 days.Dept. of Biological Sciences. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis1990 .G743. Source: Masters Abstracts International, Volume: 30-03, page: 0654. Thesis (M.Sc.)--University of Windsor (Canada), 1989.
Greaves, Douglas Standish., "Age related changes and tissue distribution of parvalbumin in both normal and dystrophic 129 ReJ mice." (1989). Electronic Theses and Dissertations. 913.