Title
Utilization of stomach content DNA to determine diet diversity in piscivorous fishes
Document Type
Article
Publication Date
2011
Publication Title
Journal of Fish Biology
Volume
78
Issue
4
First Page
1170
Last Page
1182
Abstract
The objective of the study was to validate and apply DNA-based approaches to describe fish diets. Laboratory experiments were performed to determine the number of hours after ingestion that DNA could be reliably isolated from stomach content residues, particularly with small prey fishes (c. 1 cm, < 0 center dot 75 g). Additionally, experiments were conducted at different temperatures to resolve temperature effects on digestion rate and DNA viability. The molecular protocol of cloning and sequencing was then applied to the analysis of stomach contents of wild fishes collected from the western basin of Lake Erie, Canada-U.S.A. The results showed that molecular techniques were more precise than traditional visual inspection and could provide insight into diet preferences for even highly digested prey that have lost all physical characteristics.
DOI
10.1111/j.1095-8649.2011.02925.x
Recommended Citation
Carreon-Martinez, L.; Johnson, T. B.; Ludsin, S. A.; and Heath, Daniel D.. (2011). Utilization of stomach content DNA to determine diet diversity in piscivorous fishes. Journal of Fish Biology, 78 (4), 1170-1182.
https://scholar.uwindsor.ca/glierpub/63
Comments
This is an accepted manuscript version of an aritcle whose version of record was published in:Journal of Fish Biology: http://dx.doi.org/10.1111/j.1095-8649.2011.02925.x