Title
Isolation and structure of an acetolactate synthase gene from Schizosaccharomyces pombe and complementation of the ilv2 mutation in Saccharomyces cerevisiae
Document Type
Article
Publication Date
1993
Publication Title
Current Genetics
Volume
24
Issue
6
First Page
544
Last Page
547
Abstract
A gene encoding a functional acetolactate synthase (ALS) subunit has been isolated from the fission yeast Schizosaccharomyces pombe, and has been structurally and genetically characterized. The approximate 5-kbp cloned DNA segment was found to contain a 2007-bp open reading frame capable of encoding a 669 aminoacid polypeptide which exhibited 57.1% similarity to the corresponding ALS subunit from Saccharomyces cerevisiae. The putative ilv1 isolated from S. pombe was shown to encode a functional subunit of acetolactate synthase by complementation of an S. cerevisiae strain deleted for the ILV2 locus.
DOI
10.1007/BF00351720
Recommended Citation
Bekkaoui, Faouzi; Nadin-Davis, Susan A.; and Crosby, William L., "Isolation and structure of an acetolactate synthase gene from Schizosaccharomyces pombe and complementation of the ilv2 mutation in Saccharomyces cerevisiae" (1993). Current Genetics, 24, 6, 544-547.
https://scholar.uwindsor.ca/biologypub/1002