Document Type
Article
Publication Date
1993
Publication Title
Nucleic Acids Research
Volume
21
Issue
24
First Page
5782
Last Page
5785
Abstract
The use of genomic DNA-based techniques in ecological and evolutionary studies has been limited by the availability of suitable probes for species of interest due to the technical difficulty of isolating and applying such probes. We have developed a simple technique that directs polymerase chain reaction (PCR) amplification to regions rich in variable number of tandem repeats (VNTRs). By using published VNTR core sequences as primers in PCRs, fragments were amplified that showed little variation within a species, but did show differences between species. When the amplified fragments were used as probes with genomic DNA Southern blots they produced hypervariable singlelocus or few-locus patterns in fish, birds, and humans. We have named this procedure as Directed Amplification of Minisatellite-region DNA (DAMD).
DOI
10.1093/nar/21.24.5782
Recommended Citation
Heath, Daniel D.; Lwama, George K.; and Devlin, Robert H., "PCR primed with VNTR core sequences yields species specific patterns and hypervariable probes" (1993). Nucleic Acids Research, 21, 24, 5782-5785.
https://scholar.uwindsor.ca/biologypub/1106