Title
A genome-scale shRNA resource for transgenic RNAi in Drosophila
Document Type
Article
Publication Date
2011
Publication Title
Nature Methods
Volume
8
Issue
5
First Page
405
Last Page
407
Abstract
Existing transgenic RNAi resources in Drosophila melanogaster based on long double-stranded hairpin RNAs are powerful tools for functional studies, but they are ineffective in gene knockdown during oogenesis, an important model system for the study of many biological questions. We show that shRNAs, modeled on an endogenous microRNA, are extremely effective at silencing gene expression during oogenesis. We also describe our progress toward building a genome-wide shRNA resource.
DOI
10.1038/nmeth.1592
Recommended Citation
Jian-Quan Ni; Rui Zhou; Czech, Benjamin; Lu-Ping Liu; Holderbaum, Laura; Yang-Zhou, Donghui; Hye-Seok, Shim; Tao, Rong; Handler, Dominik; Karpowicz, Phillip; Binari, Richard; Booker, Matthew; Brennecke, Julius; Perkins, Lizabeth A.; Hannon, Gregory J.; and Perrimon, Norbert, "A genome-scale shRNA resource for transgenic RNAi in Drosophila" (2011). Nature Methods, 8, 5, 405-407.
https://scholar.uwindsor.ca/biologypub/880