A genome-scale shRNA resource for transgenic RNAi in Drosophila

Authors

Jian-Quan Ni
Rui Zhou
Benjamin Czech
Lu-Ping Liu
Laura Holderbaum
Donghui Yang-Zhou
Shim Hye-Seok
Rong Tao
Dominik Handler
Phillip Karpowicz, University of WindsorFollow
Richard Binari
Matthew Booker
Julius Brennecke
Lizabeth A. Perkins
Gregory J. Hannon
Norbert Perrimon

Document Type

Article

Publication Date

2011

Publication Title

Nature Methods

Volume

8

Issue

5

First Page

405

Last Page

407

Abstract

Existing transgenic RNAi resources in Drosophila melanogaster based on long double-stranded hairpin RNAs are powerful tools for functional studies, but they are ineffective in gene knockdown during oogenesis, an important model system for the study of many biological questions. We show that shRNAs, modeled on an endogenous microRNA, are extremely effective at silencing gene expression during oogenesis. We also describe our progress toward building a genome-wide shRNA resource.

DOI

10.1038/nmeth.1592

Recommended Citation

Jian-Quan Ni; Rui Zhou; Czech, Benjamin; Lu-Ping Liu; Holderbaum, Laura; Yang-Zhou, Donghui; Hye-Seok, Shim; Tao, Rong; Handler, Dominik; Karpowicz, Phillip; Binari, Richard; Booker, Matthew; Brennecke, Julius; Perkins, Lizabeth A.; Hannon, Gregory J.; and Perrimon, Norbert, "A genome-scale shRNA resource for transgenic RNAi in Drosophila" (2011). Nature Methods, 8, 5, 405-407.
https://scholar.uwindsor.ca/biologypub/880