Anaerobic induction in conifers: Expression of endogenous and chimeric anaerobically-induced genes
Document Type
Article
Publication Date
1990
Publication Title
Physiologia Plantarum
Volume
78
Issue
3
First Page
441
Last Page
446
Abstract
Alcohol dehydrogenase (ADH; EC 1.1.1.1) activity was measured in Picea glauca (Moench) Voss cell suspensions under differing conditions of hypoxia. ADH activity increased 4.5 fold after 48 h of induction. When cells were induced under different levels of hypoxia (2, 5 and 20% O2) changes in ADH activity were found to increase with lower levels of oxygen. Alanine aminotransferase (AlaAT; EC 2.6.1.2) activity increased under hypoxia in a pattern similar to ADH, however lactate dehydrogenase (LDH; EC 1.1.1.27) activity did not increase under hypoxic conditions. The ability of white spruce cells to accurately regulate heterologous anaerobic promotors was tested by electroporating chimeric ADH reporter genes into protoplasts. While protoplasts were capable of anaerobically regulating a maize ADH reporter construct, constructs with dicotyledonous promoters (pea and Arabidopsis) were not expressed.
DOI
10.1111/j.1399-3054.1990.tb09061.x
Recommended Citation
Good, A. G.; Bekkaoui, F.; Pilate, G.; Dunstan, D. I.; and Crosby, William L., "Anaerobic induction in conifers: Expression of endogenous and chimeric anaerobically-induced genes" (1990). Physiologia Plantarum, 78, 3, 441-446.
https://scholar.uwindsor.ca/biologypub/969