Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana
Document Type
Article
Publication Date
2003
Publication Title
Science
Volume
301
Issue
5633
First Page
653
Last Page
657
Abstract
Over 225,000 independent Agrobacterium transferred DNA (T-DNA) insertion events in the genome of the reference plant Arabidopsis thaliana have been created that represent near saturation of the gene space. The precise locations were determined for more than 88,000 T-DNA insertions, which resulted in the identification of mutations in more than 21,700 of the ∼29,454 predicted Arabidopsis genes. Genome-wide analysis of the distribution of integration events revealed the existence of a large integration site bias at both the chromosome and gene levels. Insertion mutations were identified in genes that are regulated in response to the plant hormone ethylene.
DOI
10.1126/science.1086391
Recommended Citation
Alonso, José M.; Stepanova, Anna N.; Leisse, Thomas J.; Kim, Christopher J.; Chen, Huaming; Shinn, Paul; Stevenson, Denise K.; Zimmerman, Justin; Barajas, Pascual; Cheuk, Rosa; Gadrinab, Carmelita; Heller, Collen; Jeske, Albert; Koesema, Eric; Meyers, Cristina C.; Parker, Holly; Prednis, Lance; Ansari, Yasser; Choy, Nathan; Deen, Hashim; Geralt, Michael; Hazari, Nisha; Hom, Emily; Karnes, Meagan; Mulholland, Celene; Ndubaku, Ral; Schmidt, Ian; Guzman, Plinio; Aguilar-Henonin, Laura; Schmid, Markus; Weigel, Detlef; Carter, David E.; Marchand, Trudy; Risseeuw, Eddy; Brogden, Debra; Zeko, Albana; Crosby, William L.; Berry, Charles C.; and Ecker, Joseph R., "Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana" (2003). Science, 301, 5633, 653-657.
https://scholar.uwindsor.ca/biologypub/988