Identification of an SCF ubiquitin–ligase complex required for auxin response in Arabidopsis thaliana

Authors

William M. Gray
J. Carlos del Pozo
Loni Walker
Lawrence Hobbie
Eddy Risseeuw
Travis Banks
William L. Crosby, University of WindsorFollow
Ming Yang
Hong Ma
Mark Estelle

Document Type

Article

Publication Date

1999

Publication Title

Genes & Development

Volume

13

Issue

13

First Page

1678

Last Page

1691

Abstract

The plant hormone auxin regulates diverse aspects of plant growth and development. We report that in Arabidopsis, auxin response is dependent on a ubiquitin-ligase (E3) complex called SCFTIR1. The complex consists of proteins related to yeast Skp1p and Cdc53p called ASK and AtCUL1, respectively, as well as the F-box protein TIR1. Mutations in either ASK1 or TIR1 result in decreased auxin response. Further, overexpression of TIR1 promotes auxin response suggesting that SCFTIR1 is limiting for the response. These results provide new support for a model in which auxin action depends on the regulated proteolysis of repressor proteins.

Recommended Citation

Gray, William M.; del Pozo, J. Carlos; Walker, Loni; Hobbie, Lawrence; Risseeuw, Eddy; Banks, Travis; Crosby, William L.; Yang, Ming; Ma, Hong; and Estelle, Mark, "Identification of an SCF ubiquitin–ligase complex required for auxin response in Arabidopsis thaliana" (1999). Genes & Development, 13, 13, 1678-1691.
https://scholar.uwindsor.ca/biologypub/995