Document Type
Article
Publication Date
1-1-2018
Publication Title
Chemical Science
Volume
9
Issue
5
First Page
1358
Last Page
1367
Abstract
Pyrophosphate (PPi) is a byproduct of DNA and RNA synthesis, and abnormal levels are indicative of disease. We report the high-affinity binding of PPi in water by N-alkyl ammonium resorcinarene chloride receptors. Experimental analysis using 1H and 31P NMR, isothermal titration calorimetry, mass spectrometry, and UV-vis spectroscopy all support exceptional selectivity of these systems for PPi in water. The measured affinity of K1 = 1.60 × 107 M-1 for PPi is three orders of magnitude larger than that observed for binding to another phosphate, ATP. This exceptional anion-binding affinity in water is explored through a detailed density functional theory computational study. These systems provide a promising avenue for the development of future innovative medical diagnostic tools.
DOI
10.1039/c7sc05167k
ISSN
20416520
E-ISSN
20416539
Recommended Citation
Beyeh, Ngong Kodiah; Díez, Isabel; Taimoory, S. Maryamdokht; Meister, Daniel; Feig, Andrew I.; Trant, John F.; Ras, Robin H.A.; and Rissanen, Kari. (2018). High-affinity and selective detection of pyrophosphate in water by a resorcinarene salt receptor. Chemical Science, 9 (5), 1358-1367.
https://scholar.uwindsor.ca/chemistrybiochemistrypub/201