Title
Time-Resolved SANS to Measure Monomer Inter-Bilayer Exchange and Intra-Bilayer Translocation
Document Type
Article
Publication Date
1-1-2022
Publication Title
Methods in Molecular Biology
Volume
2402
First Page
151
Keywords
Exchange, Flip-flop, Lipids, SANS
Last Page
161
Abstract
The monomeric exchange kinetics of sub-micron particles provide insight into their stability and dynamism. Traditional techniques used to measure the intra- and inter-particle exchange often require monitoring the transfer of bulky and perturbing fluorescent labels. Time-resolved small angle neutron scattering (TR-SANS) overcomes these flaws by isotope labeling, allowing for the monomeric exchange rate determination of unperturbed, stress-free particles. Here, we describe TR-SANS in detail and novel applications of the technique.
DOI
10.1007/978-1-0716-1843-1_13
ISSN
10643745
E-ISSN
19406029
Recommended Citation
Nguyen, Michael H.L.; DiPasquale, Mitchell; Castillo, Stuart R.; and Marquardt, Drew. (2022). Time-Resolved SANS to Measure Monomer Inter-Bilayer Exchange and Intra-Bilayer Translocation. Methods in Molecular Biology, 2402, 151-161.
https://scholar.uwindsor.ca/chemistrybiochemistrypub/288
PubMed ID
34854043