Time-Resolved SANS to Measure Monomer Inter-Bilayer Exchange and Intra-Bilayer Translocation
Methods in Molecular Biology
Exchange, Flip-flop, Lipids, SANS
The monomeric exchange kinetics of sub-micron particles provide insight into their stability and dynamism. Traditional techniques used to measure the intra- and inter-particle exchange often require monitoring the transfer of bulky and perturbing fluorescent labels. Time-resolved small angle neutron scattering (TR-SANS) overcomes these flaws by isotope labeling, allowing for the monomeric exchange rate determination of unperturbed, stress-free particles. Here, we describe TR-SANS in detail and novel applications of the technique.
Nguyen, Michael H.L.; DiPasquale, Mitchell; Castillo, Stuart R.; and Marquardt, Drew. (2022). Time-Resolved SANS to Measure Monomer Inter-Bilayer Exchange and Intra-Bilayer Translocation. Methods in Molecular Biology, 2402, 151-161.