Date of Award
2006
Publication Type
Master Thesis
Degree Name
M.Sc.
Department
Biological Sciences
Keywords
Biology, Molecular.
Rights
info:eu-repo/semantics/openAccess
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Abstract
Embryos of the brine shrimp, Artemia franciscana , contain a novel cysteine protease composed of a cathepsin L-like catalytic subunit of 28.5 kDa, and a cell adhesion protein of the FAS-1 family of 31.5 kDa. The cathepsin L-like subunit is encoded by a cDNA derived from the CL-1 gene in A. franciscana which has been shown to be intron-less. The CL-1 gene was detected in genomic DNA prepared from nuclei of Artemia, and a second CL gene (CL-2) was obtained from a genomic library in EMBL3. Screening of Artemia adult and embryo cDNA libraries yielded sequences homologous to the CL-2 gene, and confirmed that CL-2 gene is also intron-less. Artemia adult CL-2 cDNA was found to contain two distinct open reading frames encoding the pro-peptide and mature protease. Several potential transcription factor binding sites were identified, indicating the possibility of a functional promoter in the 5' upstream sequence of the CL-2 gene.Dept. of Biological Sciences. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis2006 .C365. Source: Masters Abstracts International, Volume: 45-01, page: 0230. Thesis (M.Sc.)--University of Windsor (Canada), 2006.
Recommended Citation
Cao, Jian Ping, "Characterization of cathepsin L genes and their cDNAs in the brine shrimp, Artemia franciscana." (2006). Electronic Theses and Dissertations. 1398.
https://scholar.uwindsor.ca/etd/1398
