"NEW FLUOROMETRIC METHODS FOR THE DETERMINATION OF ALUMINUM, PYROPHOSPH" by NARACE DYAL. SEUDEAL

Date of Award

1987

Publication Type

Doctoral Thesis

Degree Name

Ph.D.

Department

Chemistry and Biochemistry

Keywords

Chemistry, Biochemistry.

Rights

info:eu-repo/semantics/openAccess

Abstract

A technique has been developed to determine ATP and pyrophosphate from one aliquot of platelets. The sample which contains both ATP and pyrophosphate was used to generate NAD$\sp{+}$ through a sequence of enzymatic reactions starting with ATP. The NAD$\sp{+}$ generated was condensed with acetone to form a fluorescent product. Prior to the formation of this product, pyrophosphate was separated from NAD$\sp{+}$ by passage of the reaction mixture through a Dowex-1 (Cl$\sp{-}$) resin and subsequent elution with HCl. Recoveries for both assays ranged from 98-106%, and coefficients of variation for both within-run and between-run studies for both assays were below 7%.Dept. of Chemistry and Biochemistry. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis1987 .S387. Source: Dissertation Abstracts International, Volume: 48-10, Section: B, page: 2962. Thesis (Ph.D.)--University of Windsor (Canada), 1987.

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