Date of Award

2010

Publication Type

Master Thesis

Degree Name

M.Sc.

Department

Chemistry and Biochemistry

Keywords

Biochemistry.

Supervisor

Mutus, Bulent (Chemistry and Biochemistry)

Rights

info:eu-repo/semantics/openAccess

Abstract

Platelet aggregation is a complex process that results in side effects for patients with diabetes, atherosclerosis, stroke and heart attack. Although thoroughly studied, the details of the aggregation cascade are not determined. The mechanism for participation of protein disulfide isomerase (PDI) in platelet function has received attention in literature however has not been established. In addition, heat shock protein 70 (Hsp70) was found on the platelet surface and was investigated for its role in platelet aggregation. Thymosin was of interest as a potential effector in platelet aggregation. An additional goal of the study was further optimization of the flow cell chamber platelet aggregation model. UV-VIS Spectrophotometric and flow cell chamber data that indicate no significant effect of excess platelet surface PDI, b-b or Hsp70 on platelet aggregation and adhesion. However, Thymosin has been shown to positively affect rates of platelet aggregation at the doses of 1-10 g/mL.

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