Date of Award
Great Lakes Institute for Environmental Research
Heath, Daniel (Biological Sciences)
Agriculture, Fisheries and Aquaculture.
CC BY-NC-ND 4.0
An analysis of a library consisting of major histocompatibility (MH) class I A1 and class II B1 sequences for Chinook salmon (Oncorhynchus tshawytscha) identified mutation clusters or "hotspots" in the sequence, as well as identical mutations occurring independently. This suggests that the MH genes in Chinook salmon undergo somatic mutation. In sperm competition experiments jacks sired a higher proportion of eggs than hooknose males. Furthermore, an analysis of variance indicated that cryptic female choice had occurred. To further investigate this, I genotyped parents and offspring at the MH class I and class II peptide binding region to determine whether the differential fertilization success is driven by variation in genetic compatibility between the ova and sperm. I found that mate choice for the MH class I gene had not occurred; however, jack sperm that were more similar at the MH class II gene to the female had higher fertilization success.
Helou, Leila, "The Major Histocompatibility (MH) Genes: Somatic Mutation and Mate Choice in Chinook Salmon (Oncorhynchus Tshawytscha)" (2010). Electronic Theses and Dissertations. 365.