Date of Award

2009

Publication Type

Master Thesis

Degree Name

M.Sc.

Department

Chemistry and Biochemistry

First Advisor

Bulent Mutus

Keywords

Pure sciences

Rights

info:eu-repo/semantics/openAccess

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Abstract

The first project studied the three nitric oxide synthases under different oxygen tensions. We found that eNOS can reduce NO2- to NO in hypoxia and anoxia, using endothelial cells and isolated enzymes. This suggests a new role for eNOS in times of restricted oxygen.

In the next project, gold nanoparticles were joined with a disulfide bond-bearing crosslinker to create a blue probe that turned red upon reduction with low molecular weight thiols. The rate depended on the size of the molecule. Since small thiols help control the redox of proteins their quantification helps identify oxidative stress.

In the third project, seven proteins isolated by gold nanoparticles were identified; these may have bound by their surface thiols. Additionally, gold nanoparticles were used to S-denitrosylate GSNO, and a flow device was designed that would automate this method. The prototype model is manually operated but shows potential to be developed for several applications.

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