Title
Isolation and characterization of microsatellite loci in the redside dace, Clinostomus elongatus
Document Type
Article
Publication Date
2009
Publication Title
Conservation Genetics Resources
Volume
1
Issue
1
First Page
381
Keywords
Primers, Population genetics, Paternity, Conservation, Mating system
Last Page
383
Abstract
We isolated and characterized eight polymorphic microsatellite loci for redside dace (Clinostomus elongatus), a colorful North American cyprinid that is threatened or endangered throughout most of its range. The number of alleles per locus ranged from three to eighteen, with observed heterozygosity ranging from 0.31-0.92. Cross-amplification revealed that these markers will also be useful for examining closely related and more distantly related species, including the rosyside dace (Clinostomus funduloides), Lahontan redside shiner (Richardsonius egregius), hornyhead chub (Nocomis biguttatus), and central stoneroller (Campostoma anomalum). These microsatellite loci will provide a valuable set of tools for examining fine and coarse scale population structure, exploring reproductive success, and testing outcomes of proposed conservation initiatives (e. g. captive breeding and translocation of wild individuals) for redside dace.
DOI
10.1007/s12686-009-9088-6
Recommended Citation
Pitcher, Trevor E.; Beneteau, Courtney L.; Walter, Ryan P.; Wilson, Chris C.; and Mandrak, Nicholas E.. (2009). Isolation and characterization of microsatellite loci in the redside dace, Clinostomus elongatus. Conservation Genetics Resources, 1 (1), 381-383.
https://scholar.uwindsor.ca/glierpub/112