PCR-based markers detect genetic variation at growth and immune function-related loci in chinook salmon (Oncorhynchus tshawytscha)

Authors

Margaret F. Docker, University of Windsor
Daniel D. Heath, University of Windsor

Document Type

Article

Publication Date

2002

Publication Title

Molecular Ecology Notes

Volume

2

First Page

606

Keywords

growth hormone introns, insulin-like growth factor, MHC-B1, Oncorhynchus tshawytscha, PCR-RFLP, transferrin

Last Page

609

Abstract

We developed seven polymerase chain reaction (PCR) based markers that detect genetic variation at loci related to growth (four) and immune function (three) in chinook salmon. One assay shows a length polymorphism following PCR; the others show restriction fragment length polymorphisms (PCR-RFLP). Two alleles were detected in each assay, and the common alleles were found at frequencies of 0.67-0.95 in seven wild populations in British Columbia, Canada. These loci also amplified in other salmonid species. These markers, by detecting variation linked to genes with high fitness relevance, are expected to be useful in a range of theoretical and applied studies.

DOI

10.1046/j.1471-8278 .2002.00315.x

Recommended Citation

Docker, Margaret F. and Heath, Daniel D.. (2002). PCR-based markers detect genetic variation at growth and immune function-related loci in chinook salmon (Oncorhynchus tshawytscha). Molecular Ecology Notes, 2, 606-609.
https://scholar.uwindsor.ca/glierpub/157