PCR-RFLP markers detect 29 mitochondrial haplotypes in Pacific lamprey (Entosphenus tridentatus)

Authors

M. F. Docker, University of Windsor
G. R. Haas, University of Alaska Fairbanks
H. Goodman, Humboldt State University
S. B. Reids
D. D. Heath, University of Windsor

Document Type

Article

Publication Date

2007

Publication Title

Molecular Ecology Notes

Volume

7

First Page

350

Keywords

Entosphenus tridentatus, genetic variation, Lampetra tridentata, mitochondrial DNA, Pacific lamprey, PCR-RFLP

Last Page

353

Abstract

We developed five polymerase chain reaction-based markers that detect variation in the mitochondrial genome of the Pacific lamprey, Entosphenus tridentatus, across most of its range. Two gene fragments (ND2 and ND5) were amplified and digested with three and two restriction enzymes, respectively, detecting sequence variation at 18 sites (ND2 = 13; ND5 = 5) and yielding 29 composite haplotypes among 1246 lampreys. These sequencebased markers will be useful in a range of phylogeographical and population genetic studies.

DOI

10.1111/j.1471-8286.2006.01605.x

Recommended Citation

Docker, M. F.; Haas, G. R.; Goodman, H.; Reids, S. B.; and Heath, D. D.. (2007). PCR-RFLP markers detect 29 mitochondrial haplotypes in Pacific lamprey (Entosphenus tridentatus). Molecular Ecology Notes, 7, 350-353.
https://scholar.uwindsor.ca/glierpub/168