Document Type

Article

Publication Date

2007

Publication Title

Molecular Ecology Notes

Volume

7

First Page

350

Last Page

353

DOI

10.1111/j.1471-8286.2006.01605.x

Keywords

Entosphenus tridentatus, genetic variation, Lampetra tridentata, mitochondrial DNA, Pacific lamprey, PCR-RFLP

Abstract

We developed five polymerase chain reaction-based markers that detect variation in the mitochondrial genome of the Pacific lamprey, Entosphenus tridentatus, across most of its range. Two gene fragments (ND2 and ND5) were amplified and digested with three and two restriction enzymes, respectively, detecting sequence variation at 18 sites (ND2 = 13; ND5 = 5) and yielding 29 composite haplotypes among 1246 lampreys. These sequencebased markers will be useful in a range of phylogeographical and population genetic studies.

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