Characterization of one trinucleotide and six dinucleotide microsatellite markers in bicolor damselfish, Stegastes partitus, a common coral reef fish

Authors

Roger J. Thiessen, University of Windsor
Daniel D. Heath, University of Windsor

Document Type

Article

Publication Date

2007

Publication Title

Conservation Genetics

Volume

8

First Page

983

Keywords

Stegastes partitus, Stegastes leucostictus, Stegastes planifrons, Stegastes adustus, Microspathodon chrysurus

Last Page

985

Abstract

Fourteen primer pairs were designed from 47 sequences containing microsatellites isolated from a genomic library enriched for (GACA)4 in Stegastes partitus. Seven of these were shown to be polymorphic and provided clean amplification products. These seven loci were further characterized using 38 individuals, yielding a range in number of alleles from 5 to 31 and observed and expected heterozygosity values ranging from 0.45–0.89 and 0.66–0.96 respectively. Six of seven loci conformed to Hardy-Weinberg equilibrium. Successful amplification was also achieved in other Pomacentridae.

DOI

10.1007/s10592-006-9207-9

Recommended Citation

Thiessen, Roger J. and Heath, Daniel D.. (2007). Characterization of one trinucleotide and six dinucleotide microsatellite markers in bicolor damselfish, Stegastes partitus, a common coral reef fish. Conservation Genetics, 8, 983-985.
https://scholar.uwindsor.ca/glierpub/169