Characterization of eight polymorphic microsatellite DNA markers for the greenside darter, Etheostoma blennioides (Percidae)

Authors

Courtney L. Beneteau, University of Windsor
Nicholas E. Mandrak, Fisheries and Oceans Canada
Daniel D. Heath, University of Windsor

Document Type

Article

Publication Date

2007

Publication Title

Molecular Ecology Notes

Volume

7

First Page

641

Keywords

Etheostoma, genetic structure, greenside darter, microsatellites, Percidae

Last Page

643

Abstract

The greenside darter, Etheostoma blennioides is a small benthic fish found in fast-flowing streams in eastern North America. In Canada, this species is native to three, and introduced into one, Great Lakes tributaries in southwestern Ontario. It is currently listed as a species of Special Concern. To characterize population genetic structure and diversity in the Canadian populations of greenside darter, eight polymorphic microsatellite markers were developed for the species. The polymerase chain reaction primers were tested between 32 and 60 individuals from the Sydenham River and yielded a high number of alleles (four to 42 per locus), and observed heterozygosities ranging from 0.14 to 0.82.

DOI

10.1111/j.1471-8286.2006.01661.x

Recommended Citation

Beneteau, Courtney L.; Mandrak, Nicholas E.; and Heath, Daniel D.. (2007). Characterization of eight polymorphic microsatellite DNA markers for the greenside darter, Etheostoma blennioides (Percidae). Molecular Ecology Notes, 7, 641-643.
https://scholar.uwindsor.ca/glierpub/171