Expansion of the global RNA virome reveals diverse clades of bacteriophages
Document Type
Article
Publication Date
10-13-2022
Publication Title
Cell
Volume
185
Issue
21
First Page
4023
Keywords
Bactriophage, Functional protein annotation, Metatranscriptomics, RNA dependent RNA polymerase, RNA Virus, Viral Ecology, viral phylogeny, Virus, Virus - Host prediction
Last Page
4037.e18
Abstract
High-throughput RNA sequencing offers broad opportunities to explore the Earth RNA virome. Mining 5,150 diverse metatranscriptomes uncovered >2.5 million RNA virus contigs. Analysis of >330,000 RNA-dependent RNA polymerases (RdRPs) shows that this expansion corresponds to a 5-fold increase of the known RNA virus diversity. Gene content analysis revealed multiple protein domains previously not found in RNA viruses and implicated in virus-host interactions. Extended RdRP phylogeny supports the monophyly of the five established phyla and reveals two putative additional bacteriophage phyla and numerous putative additional classes and orders. The dramatically expanded phylum Lenarviricota, consisting of bacterial and related eukaryotic viruses, now accounts for a third of the RNA virome. Identification of CRISPR spacer matches and bacteriolytic proteins suggests that subsets of picobirnaviruses and partitiviruses, previously associated with eukaryotes, infect prokaryotic hosts.
DOI
10.1016/j.cell.2022.08.023
ISSN
00928674
E-ISSN
10974172
Recommended Citation
Neri, Uri; Wolf, Yuri I.; Roux, Simon; Camargo, Antonio Pedro; Lee, Benjamin; Kazlauskas, Darius; Chen, I. Min; Ivanova, Natalia; Zeigler Allen, Lisa; Paez-Espino, David; Bryant, Donald A.; Bhaya, Devaki; Narrowe, Adrienne B.; Probst, Alexander J.; Sczyrba, Alexander; Kohler, Annegret; Séguin, Armand; and Shade, Ashley. (2022). Expansion of the global RNA virome reveals diverse clades of bacteriophages. Cell, 185 (21), 4023-4037.e18.
https://scholar.uwindsor.ca/glierpub/527
PubMed ID
36174579