Metagenomics of Wastewater Influent from Wastewater Treatment Facilities across Ontario in the Era of Emerging SARS-CoV-2 Variants of Concern

Authors

Opeyemi U. Lawal, Ontario Veterinary College
Linkang Zhang, Ontario Veterinary College
Valeria R. Parreira, Ontario Veterinary College
R. Stephen Brown, Queen’s University
Charles Chettleburgh, Ontario Agricultural College
Nora Dannah, Toronto Metropolitan University
Robert Delatolla, University of Ottawa
Kimberly A. Gilbride, Toronto Metropolitan University
Tyson E. Graber, Children's Hospital of Eastern Ontario, Ottawa
Golam Islam, Ontario Tech University
James Knockleby, Health Sciences North Research Institute
Sean Ma, Ontario Agricultural College
Hanlan McDougall, Ontario Agricultural College
R. Michael McKay, Great Lakes Institute for Environmental Research
Aleksandra Mloszewska, Health Sciences North Research Institute
Claire Oswald, Toronto Metropolitan University
Mark Servos, University of Waterloo
Megan Swinwood-Sky, Ontario Agricultural College

Document Type

Article

Publication Date

7-1-2022

Publication Title

Microbiology Resource Announcements

Volume

11

Issue

7

Abstract

We report metagenomic sequencing analyses of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in composite wastewater influent from 10 regions in Ontario, Canada, during the transition between Delta and Omicron variants of concern. The Delta and Omicron BA.1/BA.1.1 and BA.2-defining mutations occurring in various frequencies were reported in the consensus and subconsensus sequences of the composite samples.

DOI

10.1128/mra.00362-22

E-ISSN

2576098X

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Recommended Citation

Lawal, Opeyemi U.; Zhang, Linkang; Parreira, Valeria R.; Brown, R. Stephen; Chettleburgh, Charles; Dannah, Nora; Delatolla, Robert; Gilbride, Kimberly A.; Graber, Tyson E.; Islam, Golam; Knockleby, James; Ma, Sean; McDougall, Hanlan; McKay, R. Michael; Mloszewska, Aleksandra; Oswald, Claire; Servos, Mark; and Swinwood-Sky, Megan. (2022). Metagenomics of Wastewater Influent from Wastewater Treatment Facilities across Ontario in the Era of Emerging SARS-CoV-2 Variants of Concern. Microbiology Resource Announcements, 11 (7).
https://scholar.uwindsor.ca/glierpub/530