Document Type
Article
Publication Date
1-1-2017
Publication Title
Applied and Environmental Microbiology
Volume
83
Issue
2
Keywords
Antagonistic, Kynurenine, Pseudomonads, Pseudomonas aeruginosa, Siderophore, Thioquinolobactin
Abstract
Pseudomonas aeruginosa is an opportunistic pathogen which is evolving resistance to many currently used antibiotics. While much research has been devoted to the roles of pathogenic P. aeruginosa in cystic fibrosis (CF) patients, less is known of its ecological properties. P. aeruginosa dominates the lungs during chronic infection in CF patients, yet its abundance in some environments is less than that of other diverse groups of pseudomonads. Here, we sought to determine if clinical isolates of P. aeruginosa are vulnerable to environmental pseudomonads that dominate soil and water habitats in one-to-one competitions which may provide a source of inhibitory factors. We isolated a total of 330 pseudomonads from diverse habitats of soil and freshwater ecosystems and competed these strains against one another to determine their capacity for antagonistic activity. Over 900 individual inhibitory events were observed. Extending the analysis to P. aeruginosa isolates revealed that clinical isolates, including ones with increased alginate production, were susceptible to competition by multiple environmental strains. We performed transposon mutagenesis on one isolate and identified an ~14.8-kb locus involved in antagonistic activity. Only two other environmental isolates were observed to carry the locus, suggesting the presence of additional unique compounds or interactions among other isolates involved in outcompeting P. aeruginosa. This collection of strains represents a source of compounds that are active against multiple pathogenic strains. With the evolution of resistance of P. aeruginosa to currently used antibiotics, these environmental strains provide opportunities for novel compound discovery against drug-resistant clinical strains.
DOI
10.1128/AEM.02701-16
ISSN
00992240
E-ISSN
10985336
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Chatterjee, Payel; Davis, Elizabeth; Yu, Fengan; James, Sarah; Wildschutte, Julia H.; Wiegmann, Daniel D.; Sherman, David H.; McKay, Robert M.; LiPuma, John J.; and Wildschutte, Hans. (2017). Environmental pseudomonads inhibit cystic fibrosis patient-derived Pseudomonas aeruginosa. Applied and Environmental Microbiology, 83 (2).
https://scholar.uwindsor.ca/glierpub/561
PubMed ID
27881418
Included in
Biochemistry, Biophysics, and Structural Biology Commons, Biodiversity Commons, Biology Commons, Marine Biology Commons