Cyanobacterial bioreporters as sensors of nutrient availability

Document Type

Article

Publication Date

8-18-2010

Publication Title

Advances in Biochemical Engineering/Biotechnology

Volume

118

First Page

165

Keywords

Bioreporter, Cyanobacteria, Luciferase, Nutrients, Synechococcus

Last Page

188

Abstract

Due to their ubiquity in aquatic environments and their contribution to total biomass, especially in oligotrophic systems, cyanobacteria can be viewed as a proxy for primary productivity in both marine and fresh waters. In this chapter we describe the development and use of picocyanobacterial bioreporters to measure the bioavailability of nutrients that may constrain total photosynthesis in both lacustrine and marine systems. Issues pertaining to bioreporter construction, performance and field applications are discussed. Specifically, luminescent Synechococcus spp. and Synechocystis spp. bioreporters are described that allow the bioavailability of phosphorus, nitrogen and iron to be accurately measured in environmental samples. © Springer-Verlag Berlin Heidelberg 2010.

DOI

10.1007/10_2009_23

ISSN

07246145

ISBN

9783642008641

PubMed ID

20091289

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