Document Type
Article
Publication Date
1-1-2022
Publication Title
Methods in Molecular Biology
Volume
2402
First Page
163
Keywords
Contrast-matching, Domains, Lipid rafts, Lipid vesicles, Phase separation, Small angle neutron scattering
Last Page
177
Abstract
Lipid domains in model membranes are routinely studied to provide insight into the physical interactions that drive raft formation in cellular membranes. Using small angle neutron scattering, contrast-matching techniques enable the detection of lipid domains ranging from tens to hundreds of nanometers which are not accessible to other techniques without the use of extrinsic probes. Here, we describe a probe-free experimental approach and model-free analysis to identify lipid domains in freely floating vesicles of ternary phase separating lipid mixtures.
DOI
10.1007/978-1-0716-1843-1_14
ISSN
10643745
E-ISSN
19406029
Recommended Citation
DiPasquale, Mitchell; Nguyen, Michael H.L.; Castillo, Stuart R.; Heberle, Frederick A.; and Marquardt, Drew. (2022). Identifying Membrane Lateral Organization by Contrast-Matched Small Angle Neutron Scattering. Methods in Molecular Biology, 2402, 163-177.
https://scholar.uwindsor.ca/chemistrybiochemistrypub/289
PubMed ID
34854044