Identifying Membrane Lateral Organization by Contrast-Matched Small Angle Neutron Scattering

Authors

Mitchell DiPasquale, University of Windsor
Michael H.L. Nguyen, University of Windsor
Stuart R. Castillo, University of Windsor
Frederick A. Heberle, The University of Tennessee, Knoxville
Drew Marquardt, University of Windsor

Document Type

Article

Publication Date

1-1-2022

Publication Title

Methods in Molecular Biology

Volume

2402

First Page

163

Keywords

Contrast-matching, Domains, Lipid rafts, Lipid vesicles, Phase separation, Small angle neutron scattering

Last Page

177

Abstract

Lipid domains in model membranes are routinely studied to provide insight into the physical interactions that drive raft formation in cellular membranes. Using small angle neutron scattering, contrast-matching techniques enable the detection of lipid domains ranging from tens to hundreds of nanometers which are not accessible to other techniques without the use of extrinsic probes. Here, we describe a probe-free experimental approach and model-free analysis to identify lipid domains in freely floating vesicles of ternary phase separating lipid mixtures.

DOI

10.1007/978-1-0716-1843-1_14

ISSN

10643745

E-ISSN

19406029

Recommended Citation

DiPasquale, Mitchell; Nguyen, Michael H.L.; Castillo, Stuart R.; Heberle, Frederick A.; and Marquardt, Drew. (2022). Identifying Membrane Lateral Organization by Contrast-Matched Small Angle Neutron Scattering. Methods in Molecular Biology, 2402, 163-177.
https://scholar.uwindsor.ca/chemistrybiochemistrypub/289

PubMed ID

34854044