Document Type
Article
Publication Date
2010
Publication Title
Applied Optics
Volume
49
Issue
13
First Page
C27
Last Page
C35
Abstract
Laser-induced breakdown spectroscopy has been utilized to classify and identify bacterial specimens on the basis of their atomic composition. We have characterized the effect that the presence of a second bacterial species in the ablated specimen had on the identification of the majority species. Specimens with a reduced number of bacterial cells (approximately 2500) were identified with 100% accuracy when compared to undiluted specimens. In addition, a linear dependence of the total spectral power as a function of cell number was determined. Lastly, a high selectivity was obtained for a LIBS-based analysis of nine separate bacterial strains from four genera.
Recommended Citation
Rehse, Steven J.; Mohaidat, Q.I.; and Palchaudhuri, S.. (2010). Towards the clinical application of laser-induced breakdown spectroscopy for rapid pathogen diagnosis: The effect of mixed cultures and sample dilution on bacterial identification. Applied Optics, 49 (13), C27-C35.
https://scholar.uwindsor.ca/physicspub/30
Comments
This paper was published in Applied Optics and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://dx.doi.org/10.1364/AO.49.000C27. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.