Development of a gold nanoparticle sensor to detect environmental DNA of invasive fish species in the Great Lakes

Submitter and Co-author information

Meaghen Shiha, University of WindsorFollow

Standing

Undergraduate

Type of Proposal

Visual Presentation (Poster, Installation, Demonstration)

Faculty

Faculty of Science

Faculty Sponsor

Dr. Chitra Rangan

Proposal

The goal of my project is to develop a nano-optical sensor platform that can detect invasive fish species in the Great Lakes at the streamside. This is a collaboration between Dr. Rangan (Physics), Dr. Heath (GLIER), Dr. Pitcher (GLIER), and Dr. Mittler (Western University). I am doing preliminary experiments and simulations that can establish proof of principle so that the collaboration can seek external grant funding. I have worked on perfecting the method for creating gold nano-particle islands on glass slides using electroless deposition. With these slide sensor platforms, we will bioconjugate a recognition site (complementary eDNA) onto the nano-particles, perform a recognition reaction, and detect it using UV-Vis absorption spectroscopy. I will present the preliminary results of this interdisciplinary project related to the deposition of gold onto the glass slides.

Location

University of Windsor

Grand Challenges

Healthy Great Lakes

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Development of a gold nanoparticle sensor to detect environmental DNA of invasive fish species in the Great Lakes

University of Windsor

The goal of my project is to develop a nano-optical sensor platform that can detect invasive fish species in the Great Lakes at the streamside. This is a collaboration between Dr. Rangan (Physics), Dr. Heath (GLIER), Dr. Pitcher (GLIER), and Dr. Mittler (Western University). I am doing preliminary experiments and simulations that can establish proof of principle so that the collaboration can seek external grant funding. I have worked on perfecting the method for creating gold nano-particle islands on glass slides using electroless deposition. With these slide sensor platforms, we will bioconjugate a recognition site (complementary eDNA) onto the nano-particles, perform a recognition reaction, and detect it using UV-Vis absorption spectroscopy. I will present the preliminary results of this interdisciplinary project related to the deposition of gold onto the glass slides.