Role of Intramuscular PAI-1 During Aged Skeletal Muscle Regeneration
Standing
Undergraduate
Type of Proposal
Oral Presentation
Faculty
Faculty of Human Kinetics
Faculty Sponsor
Dr. Matthew Krause
Proposal
Role of Intramuscular PAI-1 During Aged Skeletal Muscle Regeneration S.A. Angus, F.A. Rahman & M.P. Krause Department of Kinesiology, University of Windsor, ON N9B 3P4, Canada. Primary author: anguss@uwindsor.ca Introduction: Skeletal muscle regeneration is a crucial process in returning muscle to its original function following damage. The accumulation of the extracellular matrix (ECM) is an important, yet often overlooked aspect during the regeneration process. With age, there is a decline in the regenerative capacity of skeletal muscle, partly attributed to the accumulation of the ECM, called fibrosis. Therefore, investigation into the contributors of fibrosis, namely plasminogen activator inhibitor-1 (PAI-1), is needed to better understand changes in the ECM during regeneration in aged muscle.
Methods: Young (3 month old) and aged (18 month old) male C57BL/6J mice were studied (n = 15). Muscle damage was induced via cardiotoxin (CTX) injection to the left tibialis anterior muscle. Mice were sacrificed at 3, 5 and 7 days following damage and muscles were collected. Immunohistochemical (IHC) techniques were used to investigate regenerative capacity, macrophage density, and macrophage-derived PAI-1 expression. A two-way ANOVA was used to determine statistical significance for the results.
Results: Myogenic potential of aged skeletal muscle was found to be significantly lower in the 7-day post-damage compared to young (main effect: p < 0.05). In addition, a significant increase in macrophage count at 7-day post-damage in aged skeletal muscle was observed. There was a significant difference in macrophage-derived PAI-1 content throughout the regeneration process between groups (p < 0.05). Discussion: Aged skeletal muscle was observed to have a diminished regenerative capacity as well as an increase in prolonged necrosis following damage. These findings suggest that the implications of macrophage-derived PAI-1 may have a negative effect on the ECM and the regenerative capacity of aged skeletal muscle.
Location
University of Windsor
Grand Challenges
Viable, Healthy and Safe Communities
Role of Intramuscular PAI-1 During Aged Skeletal Muscle Regeneration
University of Windsor
Role of Intramuscular PAI-1 During Aged Skeletal Muscle Regeneration S.A. Angus, F.A. Rahman & M.P. Krause Department of Kinesiology, University of Windsor, ON N9B 3P4, Canada. Primary author: anguss@uwindsor.ca Introduction: Skeletal muscle regeneration is a crucial process in returning muscle to its original function following damage. The accumulation of the extracellular matrix (ECM) is an important, yet often overlooked aspect during the regeneration process. With age, there is a decline in the regenerative capacity of skeletal muscle, partly attributed to the accumulation of the ECM, called fibrosis. Therefore, investigation into the contributors of fibrosis, namely plasminogen activator inhibitor-1 (PAI-1), is needed to better understand changes in the ECM during regeneration in aged muscle.
Methods: Young (3 month old) and aged (18 month old) male C57BL/6J mice were studied (n = 15). Muscle damage was induced via cardiotoxin (CTX) injection to the left tibialis anterior muscle. Mice were sacrificed at 3, 5 and 7 days following damage and muscles were collected. Immunohistochemical (IHC) techniques were used to investigate regenerative capacity, macrophage density, and macrophage-derived PAI-1 expression. A two-way ANOVA was used to determine statistical significance for the results.
Results: Myogenic potential of aged skeletal muscle was found to be significantly lower in the 7-day post-damage compared to young (main effect: p < 0.05). In addition, a significant increase in macrophage count at 7-day post-damage in aged skeletal muscle was observed. There was a significant difference in macrophage-derived PAI-1 content throughout the regeneration process between groups (p < 0.05). Discussion: Aged skeletal muscle was observed to have a diminished regenerative capacity as well as an increase in prolonged necrosis following damage. These findings suggest that the implications of macrophage-derived PAI-1 may have a negative effect on the ECM and the regenerative capacity of aged skeletal muscle.