Catechin Extraction from Green Tea and Biotransformation of Catechin to Catechol in Gut Bacteria
Standing
Undergraduate
Type of Proposal
Oral Research Presentation
Challenges Theme
Open Challenge
Faculty Sponsor
John Trant
Proposal
Insulin resistance is linked to the development of many diseases including obesity, type 2 diabetes, and polycystic ovarian syndrome. The side effects associated with current treatments call to explore natural options that can improve insulin sensitivity. Green tea is known to contain rich contents of catechins; which tend to biotransform into catechol by Lactobacillus hilgardii and Lactobacillus paracasei. Catechol has been established to assist in reducing insulin resistance. Therefore, the present study aimed to optimize catechin extraction from green tea and study the bioconversion of catechin to catechol by gut bacterial strains i.e., Lactobacillus hilgardii and Lactobacillus paracasei, under optimal growth conditions. The optimization parameters of preparing green tea to obtain the most catechin include boiling time, steeping time, and solid-liquid ratio and HPLC is employed to estimate the extraction of catechins. The results of the study are employed to refine a green tea recipe to extract the maximum amount of catechin and study the conditions for catechin-catechol biotransfomation through gut bacteria.
Grand Challenges
Viable, Healthy and Safe Communities
Catechin Extraction from Green Tea and Biotransformation of Catechin to Catechol in Gut Bacteria
Insulin resistance is linked to the development of many diseases including obesity, type 2 diabetes, and polycystic ovarian syndrome. The side effects associated with current treatments call to explore natural options that can improve insulin sensitivity. Green tea is known to contain rich contents of catechins; which tend to biotransform into catechol by Lactobacillus hilgardii and Lactobacillus paracasei. Catechol has been established to assist in reducing insulin resistance. Therefore, the present study aimed to optimize catechin extraction from green tea and study the bioconversion of catechin to catechol by gut bacterial strains i.e., Lactobacillus hilgardii and Lactobacillus paracasei, under optimal growth conditions. The optimization parameters of preparing green tea to obtain the most catechin include boiling time, steeping time, and solid-liquid ratio and HPLC is employed to estimate the extraction of catechins. The results of the study are employed to refine a green tea recipe to extract the maximum amount of catechin and study the conditions for catechin-catechol biotransfomation through gut bacteria.