The Effects of Turbidity on Redside Dace (Clinostomus elongatus) Olfactory Crypt Cell Sensory Neurons
Standing
Undergraduate
Type of Proposal
Oral Research Presentation
Challenges Theme
Open Challenge
Faculty Sponsor
Dr. Barbara Zielinski
Proposal
The olfactory sensory system plays an important role in the survival of Redside dace (Clinostomus elongatus), which is an endangered teleost fish, and their population is declining due to major changes, such as deforestation, forest fires, agriculture, urbanization, and domes tic and industrial pollution. One of the consequences of these activities is increasing of water’s turbidity that can negatively affect the Redside Dace’s health, behaviour, and social interaction. In this experiment, the effects of turbidity on olfactory sensory neurons, crypt cell olfactory sensory neurons​, in the Redside Dace was examined using 200 mg/L bentonite, which is an aluminum-based clay commonly used to experimentally induce turbidity changes. The crypt cells were labelled to become visualized by applying immunohistochemistry techniques using antibodies against the calcium-binding protein S100. Investigating the impacts of turbidity on the olfactory sensory morphotypes can provide information regarding the effects of stressor situations on the olfactory neurons of fish.
Grand Challenges
Viable, Healthy and Safe Communities
The Effects of Turbidity on Redside Dace (Clinostomus elongatus) Olfactory Crypt Cell Sensory Neurons
The olfactory sensory system plays an important role in the survival of Redside dace (Clinostomus elongatus), which is an endangered teleost fish, and their population is declining due to major changes, such as deforestation, forest fires, agriculture, urbanization, and domes tic and industrial pollution. One of the consequences of these activities is increasing of water’s turbidity that can negatively affect the Redside Dace’s health, behaviour, and social interaction. In this experiment, the effects of turbidity on olfactory sensory neurons, crypt cell olfactory sensory neurons​, in the Redside Dace was examined using 200 mg/L bentonite, which is an aluminum-based clay commonly used to experimentally induce turbidity changes. The crypt cells were labelled to become visualized by applying immunohistochemistry techniques using antibodies against the calcium-binding protein S100. Investigating the impacts of turbidity on the olfactory sensory morphotypes can provide information regarding the effects of stressor situations on the olfactory neurons of fish.